张启发转基因Bt稻米的转基因Bt蛋白对老鼠血液有毒性：巴西巴西利亚大学生物科学学院遗传学与形态学系学者在《血液学与血栓栓塞性疾病》发表的科学试验研究，对转基因Bt蛋白在哺乳动物中的毒性进行了探索。他们的研究证明：转基因Bt毒素Cry1Aa, Cry1Ab、Cry 1Ac或Cry2A，及其双体，在老鼠血液中产生毒性影响，造成红血球细胞破碎。该项科学试验进一步证实了其他国家学者以前对抗虫转基因Bt蛋白造成哺乳动物细胞毒性的结论，再次颠覆了世界大规模种植转基作物约50%抗虫转基因Bt作物的“食用安全性”！同时证明：张启发及中国农业大学食品科学与营养工程学院院长罗云波院长黄昆仑教授为首团队对转基因Bt稻米“食用安全性”毒理学动物试验彻头彻尾是用“假样品”做“假试验”得“造假结论”，是极其严重学术不端行为！
Abstract: Formulated and sporulated cultures of Bacillus thuringiensis (Bt) have been widely used against insect pests, but after the advent of genetically modified plants expressing δ-endotoxins, the bioavailability of Cry proteins has been increased. For biosafety reasons their adverse effects should be studied, mainly for non-target organisms. Thus, we evaluated, in Swiss albino mice, the hematotoxicity and genotoxicity of four Bt spore-crystals genetically modified to express individually Cry1Aa, Cry1Ab, Cry1Ac, or Cry2A, administered alone by gavage with a single dose of 27 mg/ Kg, 136 mg/Kg or 270 mg/Kg, 24 h, 72 h or 7 days before euthanasia. Binary combinations of these four spore-crystal proteins were also assayed at 270 mg/Kg with a single administration 24 h before euthanasia. Control mice received filtered water or cyclophosphamide at 27 mg/kg. For hematotoxicity evaluations, blood samples were drawn by cardiac puncture and processed in a multiple automated hematology analyzer; for genotoxicity analyses, micronucleus test was carried out in mice bone marrow cells. Spore-crystal administrations provoked selective hematotoxicity for the 3 exposure times, particularly for erythroid lineage. A significant reduction in bone marrow cell proliferation demonstrated cytotoxic but not genotoxic effects. These effects persisted for all exposure times, becoming more evident at 7 days. Similar results were observed for binary combinations at 24 h, suggesting that further studies are required to clarify the mechanism involved in the hematotoxicity found in mice, and to establish the toxicological risks to non-target organisms, especially mammals, before concluding that these microbiological control agents are safe for mammals.